Photomicrography Miracles

Autofluorescence

Autofluorescence (primary fluorescence) is the fluorescence of naturally occurring substances, such as chlorophyll, collagen and fluorite. Most plant and animal tissues show some autofluorescence when excited with ultraviolet light (e.g. light of wavelength around 365 nm).

Autofluorescence microscopy

Autofluorescence microscopy is an imaging technique used to study biological samples without external fluorescent dyes. Many biological structures, like collagen, lipofuscin, and certain metabolites, naturally emit fluorescence when exposed to specific wavelengths of light. This technique is valuable in medical and biological research because it allows scientists to examine tissues and cells in their native state, reducing the risk of alteration caused by staining. Autofluorescence is particularly useful in detecting abnormalities, such as cancerous tissues, which often exhibit distinct fluorescence patterns. While it offers high-resolution imaging, background fluorescence can sometimes interfere with clarity, necessitating specialized methods to enhance signal specificity.